Genetic Data- Real-Time Polymerase Chain Reaction
MeSH ID: D060888
Description:
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction
Best practice for sharing this type of data:
Data should be shared using Real-time PCR Data Markup Language (RDML). Refer to MIQE guidelines for the essential information to be included, as well as desirable information to be shared if available.
Most suitable repositories:
Datasets should be added to the appropriate DDBJ, Genetic Testing Registry, Reference Sequence Database, ENA, NCBI Gene, NCBI Genome, Extracellular RNA Atlas, ProbeBase, or Real-Time Quantitative PCR Primer Database.
Best practice for indicating re-use of existing data:
For public datasets please provide a DOI or other stable identified for the dataset itself *and* include a citation for the dataset in the reference list. Be sure to indicate exactly which data has been re-used, particularly when multiple versions of the dataset exist. In many cases, this is best achieved by sharing the code used to extract the part of the data that you analyzed. In some cases it may be best to share the exact dataset(s) you analyzed as well.
For access-controlled data authors should provide a link to instructions for obtaining access (e.g. here is the information page for ADNI (Alzheimer's Disease Neuroimaging Initiative): http://adni.loni.usc.edu/data-samples/access-data/).
When re-using a private dataset from a previous study please contact the data owners to discuss how the data can be made public.
